ACOX1 Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB67107-50, MB67107-100

Product: Mouse IgG1. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Swiss-Prot: Q15067

Host: Mouse

Reactivity: Human

Applications: WB, IHC, IF/ICC

All Applications: WB (1/500 - 1/1000), IHC (1/50 - 1/200), IF/ICC (1/10 - 1/50)

Background: Involved in the initial and rate-limiting step of peroxisomal beta-oxidation of straight-chain saturated and unsaturated very-long-chain fatty acids.
Catalyzes the desaturation of fatty acyl-CoAs such as palmitoyl-CoA (hexadecanoyl-CoA) to 2-trans-enoyl-CoAs ((2E)-enoyl-CoAs) such as (2E)-hexadecenoyl-CoA, and donates electrons directly to molecular oxygen (O2), thereby producing hydrogen peroxide (H2O2).

Purification and Purity: This antibody is purified through a protein G column.

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Recognizes endogenous levels of ACOX1 protein.

Bioworld Molecular Weight: ~ 72 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of ACOX1 expression in K562 (A) whole cell lysates., Immunohistochemical analysis of ACOX1 staining in human colon carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX., Immunofluorescent analysis of ACOX1 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Alternative Name: ACOX; Peroxisomal acyl-coenzyme A oxidase 1; AOX; Palmitoyl-CoA oxidase; Straight-chain acyl-CoA oxidase; SCOX

Immunogen: Recombinant fusion protein of human ACOX1. The exact sequence is proprietary.

Conjugate: Unconjugated

Modification: Unmodified