APAF1 Polyclonal Antibody

Sizes: 60μL, 120μL, 200μL

Catalogue Numbers: E-AB-12215-60, E-AB-12215-120, E-AB-12215-200

Citations, Manuals and MSDS Available upon request.

Abbreviation: APAF1

Target Synonym: APAF 1; Apaf-1; APAF; Apaf1; Apoptotic peptidase activating factor 1; Apoptotic protease activating factor 1; Apoptotic protease activating factor; Apoptotic protease-activating factor 1; CED 4; CED4; KIAA0413

Research Areas: Cancer, Cell Biology, Metabolism

Conjugation: Unconjugated

Host: Rabbit

Species Reactivity: Human, Mouse, Rat

Application: WB, IHC, ELISA

Isotype: IgG

Clonality: Polyclonal

UNIProt ID: O14727

Background: This gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms.

Concentration: 0.7 mg/mL

Immunogen: Synthetic peptide of human APAF1

Buffer: PBS with 0.05% sodium azide and 50% glycerol, PH7.4

Purification Method: Affinity purification

Dilution: WB 1:500-1:2000, IHC 1:50-1:200

Calculated MW: 142kDa

Storage: -20°C/One year. Avoid freeze / thaw cycles.

Shipping: Ice bag

Research Use Only