Human sVEGFR-1 (soluble Vascular Endothelial Growth Factor Receptor 1) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK8719-48, ELK8719-96, ELK8719-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: Svegfr-1

Assay Type: Sandwich

Sensitivity: 3 pg/mL

Standard: 500 pg/mL

Detection Range: 7.82-500 pg/mL

Sample Type: serum, plasma, tissue homogenates, saliva, cell lysates, follicular fluid and other biological fluids

Assay Length: 3.5h

Research Areas: Signal transduction; Tumor immunity;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human sVEGFR-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human sVEGFR-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human sVEGFR-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human sVEGFR-1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only