EasyStep Human SAA (Serum Amyloid A) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK042ES-48, ELK042ES-96, ELK042ES-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: SA-A; SAA1; PIG4; TP53I4; Amyloid protein A; Amyloid fibril protein AA; Serum amyloid A-1 protein

Assay Type: Sandwich

Sensitivity: 0.15 ng/mL

Standard: 50 ng/mL

Detection Range: 0.78-50 ng/mL

Sample Type: Serum, plasma

Assay Length: 1.5h

Research Areas: Infection immunity; Kidney biomarker; Hepatology; Urology;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human SAA, and the Human SAA standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human SAA. After TMB substrate solution is added, only those wells that contain Human SAA and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SAA in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: P0DJI8

Research Use Only