Recombinant Human GBA3/CBGL1 Protein (His Tag)(Active)

Size: 50μg

Catalogue Number: PKSH031073-50

Citations, Manuals and MSDS Available upon request.

Abbreviation: GBA3; CBGL1

Target Symptom: CBG; CBGL1; GBA3; GLUC; KLrP; MGC104276; MGC126878

Research Area: Signal Transduction; metabolism;

Target Species: Human

Expression Host: Baculovirus-Insect Cells

Application: Enzyme

Fusion Tag: C-His

Accession: NP_066024.1

Background: Cytosolic beta-glucosidase, also known as Cytosolic beta-glucosidase-like protein 1, GBA3, CBG and CBGL1 is a cytoplasm protein which belongs to theglycosyl hydrolase 1 family and Klotho subfamily. GBA3 / CBGL1 is a glycosidase probably involved in the intestinal absorption and metabolism of dietary flavonoid glycosides. GBA3 / CBGL1 is present in small intestine (at protein level). GBA3 / CBGL1 is expressed in liver, small intestine, colon, spleen and kidney. GBA3 / CBGL1 is down-regulated in renal cell carcinomas and hepatocellular carcinomas. GBA3 / CBGL1 is able to hydrolyze a broad variety of glycosides including phytoestrogens, flavonols, flavones, flavanones and cyanogens. GBA3 / CBGL1 possesses beta-glycosylceramidase activity and may be involved in a nonlysosomal catabolic pathway of glycosylceramide.

Activity: Measured by its ability to hydrolyze 4-methylumbelliferyl-β-D glucopyranoside. The specific activity is > 1, 500 pmoles/min/μg.

Sequence: Met 1-Leu 469

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 55 kDa

Observed MW: 50 kDa

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Research Use Only