Phospho-Rb (Ser780) Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB11890-50, MB11890-100

Product: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Swiss-Prot: P06400

Host: Rabbit

Reactivity: Human, Rat

Applications: WB, IHC-P

All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100

Background: Cell cycle-dependent phosphorylation by a CDK inhibits Rb target binding and allows cell cycle progression. Rb inactivation and subsequent cell cycle progression likely requires an initial phosphorylation by cyclin D-CDK4/6 followed by cyclin E-CDK2 phosphorylation. Specificity of different CDK/cyclin complexes has been observed in vitro and cyclin D1 is required for Ser780 phosphorylation in vivo.

Purification and Purity: Affinity Purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: IgG

Bioworld Molecular Weight: Calculated MW: 106 kDa; Observed MW: 110 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Rb in Hela, A549, HL-60, U2OS, C6 lysates using Phospho-Rb antibody., Immunohistochemistry analysis of paraffin-embedded Human tonsil using Rb antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Alternative Name: RB1; Retinoblastoma-associated protein; p105-Rb; pRb; Rb; pp110

Immunogen: A synthetic phosphopeptide corresponding to residues surrounding Ser780 of human Rb

Conjugate: Unconjugated

Modification: Phosphorylated