Podocin Polyclonal Antibody

Catalogue Numbers: BS60405-B-50, BS60405-B-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Swiss-Prot: Q9NP85

Host: Rabbit

Reactivity: Human, Rat, Mouse

Applications: WB

All Applications: WB: 1:500~1:1000

Background: The pathological conditions of SRN1 correlate well with mutations at the NPHS2 gene, where expression of a protein known as Podocin occurs. Abnormal or inefficient signaling through Podocin protein-dependent networks contributes to the development of podocyte dysfunction and proteinuria. The human NPHS2 gene maps to chromosome 1q25-q31 and encodes a 383 amino acid protein. Podocin is an integral membrane protein that appears to fold into a hairpin-like structure with intracellular amino- and carboxy-termini. Transmembrane and cytoplasmic portions of Podocin share homology to the corresponding regions of the stomatin family proteins. Expression of high-order oligomers of Podocin in glomerular podocytes may reflect a scaffolding function that influences proper function of the glomerular filtration barrier, which is necessary for renal stability.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Podocin polyclonal antibody detects endogenous levels of Podocin protein.

Extra Notes: Western blot (WB) analysis of Podocin polyclonal antibody at 1:500 dilution
Lane1:The kidney tissue lysate of Mouse(40ug)
Lane2:PC12 whole cell lysate(40ug)
Lane3:CT-26 whole cell lysate(40ug) Lane4:HUT78 whole cell lysate(40ug) Lane5:HCT116 whole cell lysate(40ug) Lane6:HEK293T whole cell lysate(40ug)

Bioworld Molecular Weight: ~ 41 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: Podocin;NPHS2

Immunogen: Synthetic peptide, corresponding to Human Podocin.

Conjugate: Unconjugated

Modification: Unmodified