Vinculin Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB11771-50, MB11771-100

Product: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Swiss-Prot: P18206

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC-P

All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100

Background: Vinculin is a cytoskeletal protein that plays an important role in the regulation of focal adhesions and embryonic development. Three structural vinculin domains include an amino-terminal head, a short, flexible proline-rich region and a carboxy-terminal tail. In the inactive state, the head and tail domains of vinculin interact to form a closed confirmation. The open and active form of vinculin translocates to focal adhesions where it is thought to be involved in anchoring F-actin to the membrane and regulation of cell migration.

Purification and Purity: Affinity Purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: IgG

Bioworld Molecular Weight: Calculated MW: 124 kDa; Observed MW: 124 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Vinculin in K562, C6, 3T3, Hela lysates using Vinculin antibody., Immunohistochemistry analysis of paraffin-embedded Human breast cancer using Vinculin antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Alternative Name: VCL; Vinculin; Metavinculin

Immunogen: Recombinant protein of human Vinculin

Conjugate: Unconjugated

Modification: Unmodified