CD68 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS67772-50, BS67772-100

Product: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Swiss-Prot: P34810

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF/ICC

All Applications: WB (1/500 - 1/1000), IHC (1/50 - 1/200), IF/ICC (1/50 - 1/200)

Background: CD68 (macrosialin) is a heavily glycosylated transmembrane protein that is expressed by and commonly used as a marker for monocytes and macrophages. It is found on the plasma membrane, as well as endosomal and lysosomal membranes. It is proposed to bind OxLDL and has been observed as a homodimer.

Purification and Purity: The antibody was purified by immunogen affinity chromatography.

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Recognizes endogenous levels of CD68 protein.

Bioworld Molecular Weight: ~ 83 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of CD68 expression in Raji (A), mouse liver (B), rat liver (C) whole cell lysates., Immunohistochemical analysis of CD68 staining in mouse spleen formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX., Immunofluorescent analysis of CD68 staining in rat spleen cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Alternative Name: Macrosialin; Gp110; CD68

Immunogen: Recombinant protein corresponding to human CD68. The exact sequence is proprietary.

Conjugate: Unconjugated

Modification: Unmodified