KLF10 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS8200-50, BS8200-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q13118

Host: Rabbit

Reactivity: Human

Applications: WB IF

All Applications: WB: 1:500~1:2000
IF: 1:50~1:200

Background: Originally isolated from osteoblastic cells, the TGFβ-inducible early gene-1 (TIEG1) is a Krupel-like zinc finger transcription factor-encoding gene which regulates cellular growth and differentiation. TIEG1 is regulated as an early response gene by TGFβ1. It is expressed in both acinar and ductular epithelial cells from exocrine pancreas and may serve as an early response gene in pancreatic cell lines. Further, overexpression of TIEG1 in TGFβ-sensitive epithelial cells induces apoptosis. TIEG1 and EGRa are expressed from alternate promoters of the same gene. Both are highly expressed in human fetal osteoblast cells. TIEG1 is additionally expressed at high levels in PBLs, spleen and colon, and at lower levels in thymus, small intestine, ovary, prostate and skeletal muscle. The nuclear TIEG2 protein, which shares significant homology with TIEG1, was originally isolated from globin-expressing human fetal erythroid cells. TIEG2 is also expressed in fetal liver. Overexpression of TIEG2 in cultured epithelial cells inhibits cellular proliferation. TIEG2 expression is upregulated by TGFβ1 and serum deprivation.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: KLF10 polyclonal antibody detects endogenous levels of KLF10 protein.

Bioworld Molecular Weight: ~ 53 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Immunofluorescence analysis of A549 cell using KLF10 antibody. Blue: DAPI for nuclear staining.

Alternative Name: Krueppel-like factor 10; EGR-alpha; Transforming growth factor-beta-inducible early growth response protein 1; TGFB-inducible early growth response protein 1; TIEG-1; KLF10; TIEG, TIEG1

Immunogen: Recombinant full length Human KLF10.

Conjugate: Unconjugated

Modification: Unmodified