Recombinant Mouse VCAM1 Protein (His Tag)(Active) Size: 100μg Catalogue Number: PKSM040831-100 Citations, Manuals and MSDS Available upon request. Abbreviation: VCAM1 Target Symptom: CD106; Vascular cell adhesion protein 1; Vcam1; L1CAM; VCMA1; Vcam-1 Target Species: Mouse Expression Host: HEK293 Cells Application: Cell culture; ELISA Fusion Tag: C-His Accession: NP_035823.3 Background: Vascular cell adhesion molecule 1 (VCAM-1), also known as CD106, is a cell surface sialoglycoprotein belonging to the immunoglobulin superfamily. Two forms of VCAM-1 with either six or seven extracellular Ig-like domains are generated by alternative splicing, with the longer form predominant. VCAM-1 is an endothelial ligand for very late antigen-4 (VLA-4) and α 4ß 7 integrin expressed on leukocytes, and thus mediates leukocyte-endothelial cell adhesion and signal transduction. VCAM-1 expression is induced on endothelial cells during inflammatory bowel disease, atherosclerosis, allograft rejection, infection, and asthmatic responses. During these responses, VCAM-1 forms a scaffold for leukocyte migration. VCAM-1 also activates signals within endothelial cells resulting in the opening of an " endothelial cell gate" through which leukocytes migrate. VCAM-1 has been identified as a potential anti-inflammatory therapeutic target, the hypothesis being that reduced expression of VCAM-1 will slow the development of atherosclerosis. In addition, VCAM-1-activated signals in endothelial cells are regulated by cytokines indicating that it is important to consider both endothelial cell adhesion molecule expression and function during inflammatory processes. Activity: Measured by the ability of the immobilized protein to support adhesion of U937 human histiocytic lymphoma cells. When 5 x 10⁴ cells/well are added to mouse VCAM1 coated plates (10 μg/ml with 100 μl/well), approximately 70%-80% cells will adhere after 1 hour at RT. Sequence: Met 1-Glu 698 Purity: > 97 % as determined by reducing SDS-PAGE. Formulation: Lyophilized from sterile PBS, pH 7.4 Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. Reconstitution: Please refer to the printed manual for detailed information. Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method. Calculated MW: 75.8 kDa Observed MW: 90-100 kDa Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. Shipping: This product is provided as lyophilized powder which is shipped with ice packs. Research Use Only