CD35 Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB66111-50, MB66111-100

Product: Mouse IgG1. Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide.

Swiss-Prot: P17927; Q2VPA4

Host: Mouse

Reactivity: Human

Applications: IHC

All Applications: IHC (1/100 - 1/300)

Background: Complement receptor type 1`(CR1; CD35) is a type I transmembrane glycoprotein that is expressed on the surface of B cells, neutrophils, monocytes and renal podocytes. As a component of the host innate immune system, CR1/CD35 expressed on neutrophils and monocytes binds to ligands coated with the complement opsonins, C3b and C4b, which facilitates phagocytosis and production of proinflammatory cytokines. CR1/CD35 also participates in negative regulation of the complement cascade through its ability to promote dissociation of C3 and C5 convertases and by serving as one of multiple cofactors for Factor-I-mediated cleavage and inactivation of C3b and C4b.

Purification and Purity: The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Immunohistochemical analysis of CD35 staining in human follicular lymphoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6. 0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Bioworld Molecular Weight: ~ 223kDa

Specificity: Recognizes endogenous levels of CD35 protein.

Alternative Name: CR1; C3BR; Complement receptor type 1; C3b/C4b receptor; CD35; CR1L; Complement component receptor 1-like protein; Complement C4b-binding protein CR-1-like protein

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within human CD35. The exact sequence is proprietary.

Conjugate: Unconjugated

Modification: Unmodified