ERK1 Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB10890-50, MB10890-100

Product: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Swiss-Prot: P27361

Host: Rabbit

Reactivity: Human

Applications: WB, IHC-P, IP,

All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100 IP: 1/20

Background: Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements.

Purification and Purity: Affinity Purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: IgG

Bioworld Molecular Weight: Calculated MW: 43 kDa; Observed MW: 43 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of ERK1 in K562, Hela lysates using ERK1 antibody., Immunohistochemistry analysis of paraffin-embedded Human colon cancer using ERK1 antibody. High-pressure and temperature Sodium Citrate pH 6. 0 was used for antigen retrieval.

Alternative Name: MAPK3

Immunogen: A synthetic peptide of human ERK1

Conjugate: Unconjugated

Modification: Unmodified