HIF1AN Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90632-50, BS90632-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9NWT6(Human)

Host: Rabbit

Reactivity: Human

Applications: WB, ICC IHC FC

All Applications: WB:1:500-1:2,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100

Background: Factor inhibiting HIF-1 (FIH-1) exists as a homodimer and binds to HIF-1α. Specifically, FIH-1 operates as an asparaginyl hydroxylase. It catalyzes the hydroxylation of the β-carbon of Asparagine residue 803 within the carboxy terminal transactivation domain of HIF-1α. This hydroxylation event blocks the association of HIF-1α with co-activators. FIH-1 also binds to von Hippel-Lindau (VHL) tumor suppressor protein, which represses transcriptional ac-tivity of HIF-1α. In transiently transfected human osteosarcoma cells, FIH-1 localizes to the cytoplasm. The structure of FIH-1 includes a jellyroll-like β-barrel containing ferrous-binding triad residues. The gene encoding human FIH-1 maps to chromosome 10q24.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.

Specificity: HIF1AN polyclonal antibody detects endogenous levels of HIF1AN protein.

Bioworld Molecular Weight: 40 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of HIF1AN on A549 and Jurkat cell lysates using anti-HIF1AN antibody at 1/1,000 dilution., ICC staining HIF1AN in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: FIH-1, HIF1AN, FIH1, DKFZp762F1811, Factor inhibiting HIF-1, Factor inhibiting HIF1, FIH 1, FIH-1, FIH1, FLJ20615, FLJ22027, HIF1AN, HIF1N, Hypoxia inducible factor 1 alpha inhibitor, Hypoxia inducible factor 1 alpha subunit inhibitor, Hypoxia inducible factor asparagine hydroxylase, Hypoxia-inducible factor 1-alpha inhibitor, Hypoxia-inducible factor asparagine hydroxylase, Peptide aspartate beta dioxygenase,

Immunogen: Recombinant protein within human HIF1AN aa 1-200.

Conjugate: Unconjugated

Modification: Unmodified