hnRNP Q (E268) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS1885-50, BS1885-100

Sizes: 50µl, 100µl

Swiss-Prot: O60506

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

Background: Pre-mRNA splicing is a critical step in the posttranscriptional regulation of gene expression. Heterogeneous nuclear ribonucleoprotein Q (hnRNP Q) is involved in RNA processing and is necessary for efficient pre-mRNA splicing. hnRNP is widely expressed and developmentally regulated. hnRNP Q interacts with survival motor neuron protein (SMN). Loss of function of SMN results in spinal muscular atrophy, a common neurodegenerative disease. The most common deletion in SMN genes disrupts the interaction between SMN and hnRNP Q. hnRNP Q is upregulated after midnight, and this upregulation correlates with an abrupt decline in AANAT, the key enzyme in melatonin synthesis. Rhythmic AANAT mRNA degradation mediated in part by hnRNP Q implicates this enzyme in the regulation of circadian oscillation.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: hnRNP Q (E268) polyclonal antibody detects endogenous levels of hnRNP Q protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of hnRNP Q (E268) pAb at 1:500 dilution
Lane 1: U-87MG whole cell lysate (10ug)
Lane 2: HCT116 whole cell lysate (10ug)
Lane 3: C6 whole cell lysate (40ug)
Lane 4: CT26 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of hnRNP Q (E268) pAb in paraffin-embedded human tonsil carcinoma tissue at 1:50.

Bioworld Molecular Weight: ~ 65-82 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Heterogeneous nuclear ribonucleoprotein Q; hnRNP Q; Glycine- and tyrosine-rich RNA-binding protein; GRY-RBP; NS1-associated protein 1; Synaptotagmin-binding, cytoplasmic RNA-interacting protein; SYNCRIP; HNRPQ; NSAP1

Immunogen: Synthetic peptide, corresponding to amino acids 241-290 of Human hnRNP Q.

Conjugate: Unconjugated

Modification: Unmodified