NRF1 Rabbit Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB66370-50, MB66370-100

Product: Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA.

Swiss-Prot: Q16656

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF/ICC, IP,

All Applications: WB (1/500 - 1/1000), IHC (1/50 - 1/100), IF/ICC (1/50 - 1/100), IP (1/10 - 1/50)

Background: Nuclear respiratory factor 1 (NRF1) was identified as a transcription activator for the gene encoding cytochrome c. It was later found to play a role in the nuclear control of mitochondrial function . PGC-1 induces the expression of NRF1 and NRF2 . NRF1, along with the coactivator PGC-1, stimulates the promoter of mitochondrial transcription factor A, which regulates mitochondrial biogenensis and function .

Purification and Purity: The antibody was purified by immunogen affinity chromatography.

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Recognizes endogenous levels of NRF1 protein.

Bioworld Molecular Weight: ~ 68 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of NRF1 expression in C6 (A), NIH3T3 (B), Hela (C) whole cell lysates., Immunohistochemical analysis of NRF1 staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.60). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX., Immunofluorescent analysis of NRF1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.

Alternative Name: Nuclear respiratory factor 1; NRF-1; Alpha palindromic-binding protein; Alpha-pal

Immunogen: A synthetic peptide of human NRF1

Conjugate: Unconjugated

Modification: Unmodified