SATB1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS91207-50, BS91207-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q01826(Human) Q60611(Mouse)

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB, ICC FC

All Applications: WB:1:500
ICC:1:100
FC:1:50-1:100

Background: The homeoproteins CCAAT displacement protein (CDP) and special AT-rich sequence binding protein 1 (SATB1) are transcriptional repressors of many cellular genes, and they participate in cell development and cell type differentiation. SATB1 is expressed primarily in thymocytes, and, like CDP, it also contains a distinct homeobox DNA-binding domain that is essential for DNA binding. SATB1 and CDP interact through these homeodomains and synergistically function as mediators of gene expression. SATB1 contains an additional domain that has a higher affinity for DNA and specifically facilitates the direct association between SATB1 and the nuclear matrix attachment regions (MARs) of DNA. MARs are specific DNA sequences that bind to the nuclear matrix and form the base of chromosomal loops that organize the chromosomes and regulate DNA transcription and replication within the nucleus. The association of SATB1 with the core unwinding element within the base-unpairing region of MARs requires both the MAR and homeobox binding domains of SATB1.

Purification and Purity: Peptide affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: SATB1 polyclonal antibody detects endogenous levels of SATB1 protein.

Bioworld Molecular Weight: Predicted band size 86 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of SATB1 on mouse thymus tissue lysate using anti-SATB1 antibody at 1/500 dilution., ICC staining SATB1 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: SATB1, DNA binding protein SATB1, DNA-binding protein SATB1, SATB homeobox 1, SATB1, SATB1, Special AT rich sequence binding protein 1 (binds to nuclear matrix/scaffold associating DNA), Special AT rich sequence binding protein 1, Special AT-rich sequence-binding protein 1,

Immunogen: Peptide

Conjugate: Unconjugated

Modification: Unmodified