CGN Polyclonal Antibody

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Sizes: 50 µl, 100 µl

Catalogue Numbers: BS72612-50, BS72612-100

Swiss-Prot: Q9P2M7

Host: Rabbit

CGN Polyclonal Antibody-BS72612

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Reactivity: Human

Applications: WB, IHC

All Applications: WB,1:200 - 1:2000 | IHC,1:50 - 1:200

Background: Probably plays a role in the formation and regulation of the tight junction (TJ paracellular permeability barrier.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Polyclonal Antibodies

Bioworld Molecular Weight: 160kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using CGN antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s., Immunohistochemistry of paraffin-embedded Human colon carcinoma using CGN Rabbit pAb at dilution of 1:100.Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Alternative Name: CGN; cingulin

Immunogen: Recombinant fusion protein of human CGN(NP_065821.1).

Modification: Unmodified

Conjugate: Unconjugated