Desmin Rabbit Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS9895M-50, BS9895M-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P17661

Host: Rabbit

Reactivity: Human, Mouse, Rat, Zebra fish

Applications: WB, ICC/IF, IHC, FC

All Applications: WB: 1:1000-1:5000
IHC/ICC/IF: 1:50-1:200
FC: 1:50-1:100

Background: Cytoskeletal intermediate filaments (IFs) constitute a diverse group of proteins that are expressed in a highly tissue-specific manner. Intermediate filaments are constructed from two-chain alpha-helical coiled-coil molecules arranged on an imperfect helical lattice and have been widely used as markers for distinguishing individual cell types within a tissue and identifying the origins of metastatic tumors. One such intermediate filament protein, Vimentin, is a general marker of cells originating in the mesenchyme. Vimentin is frequently co-expressed with other members of the intermediate filament family such as the cytokeratins,in neoplasms including melanoma and breast carcinoma. Vimentin and Desmin, a related class III intermediate filament, are both expressed during skeletal muscle development.

Purification and Purity: Protein A affinity purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: This antibody detects endogenous levels of Desmin and does not cross-react with related proteins.

Bioworld Molecular Weight: ~ 54 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of Desmin Rabbit mAb at 1:1000 dilution
Lane 1: The skeletal muscle lysate of Human
Lane 2: The heart tissue lysate of Mouse, Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-Desmin antibody. Counter stained with hematoxylin., ICC staining Desmin in C2C12 cells (green). The nuclear counter stain is DAPI (blue).Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.

Alternative Name: DES

Immunogen: Recombinant antibody.

Conjugate: Unconjugated

Modification: Unmodified