Alix rabbit pAb

Sizes: 50μL, 100μL

Catalogue Numbers: ES18395-50, ES18395-100

Citations, Manuals and MSDS Available upon request.

Background: This gene encodes a protein that functions within the ESCRT pathway in the abscission stage of cytokinesis, in intralumenal endosomal vesicle formation, and in enveloped virus budding. Studies using mouse cells have shown that overexpression of this protein can block apoptosis. In addition, the product of this gene binds to the product of the PDCD6 gene, a protein required for apoptosis, in a calcium-dependent manner. This gene product also binds to endophilins, proteins that regulate membrane shape during endocytosis. Overexpression of this gene product and endophilins results in cytoplasmic vacuolization, which may be partly responsible for the protection against cell death. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. Related pseudogenes have been identified on chromosome 15. [provided by RefSeq, Jan 2012],

Alternate Name: Programmed cell death 6-interacting protein (PDCD6-interacting protein; ALG-2-interacting protein 1; Hp95)

Source: Rabbit

Applications: WB; ELISA

Dilution: WB 1:500-2000, ELISA (peptide)1:5000-20000

Reactivity: Human; Rat; Mouse;

Immunogen: Synthesized peptide derived from human Alix AA range: 410-490

Storage and Stability: -20°C/1 year

Clonality: Polyclonal

Isotype: IgG

Concentration: 1 mg/ml

Observed Band (KD): 85-100kD

Human Gene ID: 10015

Human SWISS Prot NO: Q8WUM4

Subcellular Location: Cytoplasm, cytosol. Melanosome. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Secreted, extracellular exosome. Cell junction, tight junction. Midbody, Midbody ring. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Colocalized with CEP55 at centrosomes of non-dividing cells. Component of the actomyosin-tight junction complex (By similarity). PDCD6IP targeting to the midbody requires the interaction with CEP55 (PubMed:18641129).

Research Use Only