NUMB Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90976-50, BS90976-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P49757(Human) Q9QZS3(Mouse) Q2LC84(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC IHC

All Applications: WB:1:1,000-1:5,000
ICC:1:50-1:200
IHC:1:50-1:100

Background: Neuronal cell fate decisions are directed in Drosophila by NUMB, a signaling adapter protein with two protein-protein interaction domains: a phosphotyrosine-binding domain and a proline-rich SH3-binding region (PRR). Mammalian NUMB homologs play a role in the determination of cell fates during development and bind with EPS15, LNX1, and NOTCH1. Conditional mouse mutants with deletion of NUMB in developing sensory ganglia show a reduction in axonal arborization in afferent fibers. Changes in cellular calcium homeostasis influences NUMB-dependent cell fate decisions during development of the nervous system. Chicken NUMB (c-NUMB) protein is localized to the basal cortex of mitotic neuroepithelial cells.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: NUMB polyclonal antibody detects endogenous levels of NUMB protein.

Bioworld Molecular Weight: 71 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of NUMB on A431 cells lysates using anti-NUMB antibody at 1/1,000 dilution., ICC staining NUMB in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: h-Numb, c14_5527, C14orf41, FLJ31314, h Numb, h-Numb, Numb, Numb homolog (Drosophila), Numb homolog, Numb protein homolog, NUMB, Protein numb homolog, Protein S171, S171,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified