L-Leucine 4-methoxy-β-naphthylamide is a substrate for aminopeptidase M and leucine aminopeptidase. Aminopeptidases are enzymes catalyzing the cleavage of amino acids from the amino terminus (N-terminus) of proteins or peptides. Aminopeptidases are distributed widely throughout the animal and plant kingdoms and are present in various subcellular organelles, in cytosol, as well as membrane components. In vitro: L-Leucine 4-methoxy-β-naphthylamide was identified as a cell-permeable substrate for aminopeptidase M and leucine aminopeptidase that was developed for intracellular analysis of protease activities . In a previous method-developing study, peptide derivatives of 4-methoxy-β-naphthylamine including L-leucine 4-methoxy-β-naphthylamide were incubated in microtiter plates together with nitrosalicylaldehyde. Selectivity was achieved by running parallel assays containing inhibitors partially selective for each of peptide derivatives. This method was successfully validated by measurements in cells isolated from cathepsin B-/--, K-/--, and L-/-- mice . In vivo: Up to now, there is no animal in vivo data reported. Clinical trial: So far, no clinical study has been conducted.

Product information

CAS Number: 4467-68-9

Molecular Weight: 322.83

Formula: C17H23ClN2O2

Chemical Name: (2S)-2-amino-N-(4-methoxynaphthalen-2-yl)-4-methylpentanamide hydrochloride

Smiles: Cl.CC(C)C[C@H](N)C(=O)NC1=CC2=CC=CC=C2C(=C1)OC

InChiKey: NECOEODWTUWPBP-RSAXXLAASA-N

InChi: InChI=1S/C17H22N2O2.ClH/c1-11(2)8-15(18)17(20)19-13-9-12-6-4-5-7-14(12)16(10-13)21-3;/h4-7,9-11,15H,8,18H2,1-3H3,(H,19,20);1H/t15-;/m0./s1

Technical Data

Appearance: Solid Power

Purity: ≥98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis

Storage Condition: Dry, dark and -20 ^oC for 1 year or refer to the Certificate of Analysis.

Shelf Life: ≥12 months if stored properly.

Stock Solution Storage: 0 - 4 ^oC for 1 month or refer to the Certificate of Analysis.

Drug Formulation: To be determined

HS Tariff Code: 382200

Products are for research use only. Not for human use.