Occludin Polyclonal Antibody Product: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Sizes: 50µl, 100µl Catalogue Numbers: BS72035-50, BS72035-100 Swiss-Prot: Q16625 Host: Rabbit Occludin Polyclonal Antibody-BS72035 Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Reactivity: Human, Mouse, Rat Applications: WB, IHC, IF ICC ELISA All Applications: WB 1:500 - 1:2000 IHC 1:100 - 1:500 IF 1:100 - 1:500 ICC 1:100 - 1:500 ELISA 1:5000 - 1:10000 Background: Occludin is an integral membrane protein closely associated with the tight junctions of epithelial and endothelial cells. Occludin is a tetraspan integral membrane protein in epithelial and endothelial tight junction (TJ) structures that can contain two extracellular loops. The protein exists in a variety of phosphorylated forms. Phosphorylation is involved in regulating both the localization and the function of occludin. Expression of occludin is up-regulated by polyunsaturated fatty acids, increasing transendothelial cell resistance and reducing cellular permeability to large molecules. The level of occludin varies greatly depending on tissue; in brain tissue, occludin is highly expressed at cell-cell contact sites. Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE). Specificity: Occludin polyclonal antibody detects endogenous levels of Occludin protein. Bioworld Molecular Weight: ~ 59 kDa Note: For research use only, not for use in diagnostic procedure. Extra Notes: Sample: Testis (Mouse) Lysate at 40 ug Kidney (Mouse) Lysate at 40 ug Primary: Anti-Occludin at 1/300 dilution, Paraformaldehyde-fixed, paraffin embedded (Normal skin)Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Occludin) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C. Alternative Name: OCLN; Occludin; BLCPMG Immunogen: KLH conjugated synthetic peptide derived from human Occludin:151-250/522 Modification: Unmodified Conjugate: Unconjugated