Mouse OB (Obestatin) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK5497-48, ELK5497-96, ELK5497-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: Obestatin

Assay Type: Competitive Inhibition

Sensitivity: 26.8 pg/mL

Standard: 5000 pg/mL

Detection Range: 78.13-5000 pg/mL

Sample Type: serum, plasma, cell lysates, cell culture supernates and other biological fluids

Assay Length: 2h

Research Areas: Metabolic pathway; Endocrinology; Hepatology; Gastroenterology; Nutrition metabolism; Hormone metabolism;

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse OB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse OB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse OB in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only