MLH1 Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB0151-50, MB0151-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P40692

Host: Mouse

Reactivity: Human

Applications: WB, ICC

All Applications: WB: 1:500~1000
ICC: 1:50~200

Background: MLH1 is an integral part of the protein complex responsible for mismatch repair that is expressed in lymphocytes, heart, colon, breast, lung, spleen, testis, prostate, thyroid and gall bladder and is methylated in several ovarian tumors. Loss of MLH1 protein expression is associated with a mutated phenotype, microsatellite instability and a predisposition to cancer. In hereditary nonpolyposis colorectal cancer (HNPCC), an autosomal dominant inherited cancer syndrome that signifies a high risk of colorectal and various other types of cancer, the MLH1 gene exhibits a pathogenic mutation. Certain cancer cell lines, including leukemia CCRF-CEM, colon HCT 116 and KM12, and ovarian cancers SK-OV-3 and IGROV-1, show complete deficiency of MLH1, while MLH1 is expressed in 60% of melanomas, 70% of noninvasive squamous cell carcinomas and 30% of invasive squamous cell carcinomas.

Purification and Purity: The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: This antibody detects endogenous levels of MLH1 and does not cross-react with related proteins

Bioworld Molecular Weight: Predicted band size: 85KDa
Observed band size: 85KDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot detection of MLH1 antibody in SW480, 293T, Jurkat and Hela cell lysates using MLH1 antibody (1:500 diluted)., Immunocytochemistry staining of HeLa cells fixed in 1% Paraformaldehyde and using anti-MLH1 antibody (dilution 1:100).

Alternative Name: DNA mismatch repair protein Mlh1; MutL protein homolog 1; COCA2

Immunogen: Recombinant full length Human MLH1.

Conjugate: Unconjugated

Modification: Unmodified