Cdx1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90289-50, BS90289-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P47902(Human)

Host: Rabbit

Reactivity: Human

Applications: WB, ICC

All Applications: WB:1:500-1:2,000
ICC:1:50-1:200

Background: The members of the murine Cdx family (Cdx1, Cdx2, and Cdx4) are members of the caudal-type homeobox family of genes, which are homologues of the Drosophila 'caudal' gene required for anterior-posterior regional identity. The intestine-specific transcription factors Cdx1 and Cdx2 are candidate genes for directing intestinal development, differentiation, proliferation and maintenance of the intestinal phenotype. The relative expression of Cdx1 to Cdx2 protein may be important in the anterior to posterior patterning of the intestinal epithelium and in defining patterns of proliferation and differentiation along the crypt-villus axis. Expression of the Cdx1 homeobox gene in epithelial intestinal cells promotes cellular growth and differentiation. Cdx1 positively regulates its own expression. Cdx1 and Cdx2 are expressed in the small intestine and colon of fetus and adult. A decrease in human Cdx1 and/or Cdx2 expression is associated with colorectal tumorigenesis. Both Cdx1 and Cdx2 genes must be expressed to reduce tumorigenic potential, to increase sensitivity to apoptosis and to reduce cell migration, suggesting that the two genes control the normal phenotype by independent pathways. The human Cdx1 gene maps to chromosome 5q31-q33 and encodes a 265-amino acid protein.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.

Specificity: Cdx1 polyclonal antibody detects endogenous levels of Cdx1 protein.

Bioworld Molecular Weight: 28 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Cdx1 on human small intestine tissue (1) and CRC cell (2) lysate using anti-Cdx1 antibody at 1/1,000 dilution., ICC staining Cdx1 in HOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: CDX1, Caudal type homeo box transcription factor 1, Caudal type homeobox 1, Caudal type homeobox protein, Caudal type homeobox protein CDX1, Caudal type homeobox transcription factor 1, Caudal-type homeobox protein 1, CDX1, CDX1, Homeobox protein CDX 1, Homeobox protein CDX-1, MGC116915, OTTHUMP00000160524,

Immunogen: Recombinant protein of full lenth sequence of human Cdx1.

Conjugate: Unconjugated

Modification: Unmodified