Bcl2 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90125-50, BS90125-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P10415(Human) P10417(Mouse)

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB, ICC FC

All Applications: WB:1:200-1:500
ICC:1:50-1:200
FC:1:50-1:100

Background: Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release.

Purification and Purity: Peptide affinity purified.

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.

Specificity: Bcl2 polyclonal antibody detects endogenous levels of Bcl2 protein.

Bioworld Molecular Weight: 22/26 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Bcl2 on different lysates using anti-Bcl2 antibody at 1/200 dilution.
Positive control:
Lane 1: HL-60
Lane 2: Mouse colon, ICC staining Bcl2 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: BCL2, Apoptosis regulator Bcl 2, Apoptosis regulator Bcl-2, Apoptosis regulator Bcl2, AW986256, B cell CLL/lymphoma 2, B cell leukemia/lymphoma 2, Bcl-2, Bcl2, BCL2, C430015F12Rik, D630044D05Rik, D830018M01Rik, Leukemia/lymphoma, B-cell, 2, Oncogene B-cell leukemia 2, PPP1R50, Protein phosphatase 1, regulatory subunit 50,

Immunogen: Synthetic peptide within human BCL-2 20-100 aa.

Conjugate: Unconjugated

Modification: Unmodified