BES1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS78135-50, BS78135-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9LN63

Host: Rabbit

Reactivity: Arabidopsis thaliana

Applications: WB

All Applications: WB,1:500 - 1:2000

Background: Encodes brassinosteroid (BR) signalling protein that accumulates in the nucleus as dephosphorylated form in response to BRs. Is phosphorylated by the BIN2 GSK3 kinase. It synergistically interacts with BIM1 to bind to E box sequences (CANNTG). The protein contains a nuclear localization signal (NLS), followed by a highly conserved amino-terminal domain (N) shared by all family members, a BIN2 phosphorylation domain (P), a PEST motif, involved in protein degradation in the absence of BR, and a carboxyl-terminal domain. BES1 can interact with the ELF6 and REF6 Jumonji N/C-domain containing proteins and may direct them to modify histone methylation upstream of some brassinosteroid responsive-genes. Works with BRAVO to regulate QC division in the root.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various tissues from Arabidopsis thaliana, using BES1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

Bioworld Molecular Weight: 43KDa

Specificity: Unmodification

Alternative Name: 107 PROTEIN; BRASSINAZOLE-RESISTANT 2; BRI1-EMS-SUPPRESSOR 1; BZR2; F18O14.7; F18O14_7

Immunogen: A synthetic peptide of arabidopsis thaliana BES1(NP_973865.1).

Conjugate: Unconjugated

Modification: Unmodified