APRT Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS66120-50, BS66120-100

Product: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Swiss-Prot: P07741

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: ELISA IHC IF ICC

All Applications: ELISA: 1:5000-1:10000
IHC: 1:100-1:500
IF: 1:100-1:500
ICC: 1:100-1:500

Background: APRT is a 180 amino acid protein that localizes to the cytoplasm and belongs to the purine/pyrimidine phosphoribosyltransferase family. Existing as a homodimer, APRT functions to catalyze the formation of inorganic pyrophosphate and AMP from adenine and 5-phosphoribosyl-1-pyrophosphate (PRPP), a reaction that is essential for both purine metabolism and AMP biosynthesis. Defects in the gene encoding APRT are the cause of APRT deficiency, also known as 2,8-dihydroxyadenine urolithiasis, which is an autosomal recessive disease that results in renal failure. The gene encoding APRT maps to human chromosome 16, which encodes over 900 genes and comprises nearly 3% of the human genome. The GAN gene is located on chromosome 16 and, with mutation, may lead to giant axonal neuropathy, a nervous system disorder characterized by increasing malfunction with growth. The rare disorder Rubinstein-Taybi syndrome is also associated with chromosome 16, as is Crohn's disease, which is a gastrointestinal inflammatory condition.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: APRT polyclonal antibody detects endogenous levels of APRT protein.

Bioworld Molecular Weight: ~ 19 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: Adenine phosphoribosyltransferase; AMP; AMP diphosphorylase; AMP pyrophosphorylase; APRT; APT_HUMAN; DKFZp686D13177; MGC125856; MGC125857; MGC129961; Transphosphoribosidase

Immunogen: KLH conjugated synthetic peptide derived from human APRT: 101-180/180

Conjugate: Unconjugated

Modification: Unmodified