IFN-α Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Catalogue Numbers: BS60294-B-50, BS60294-B-100

Sizes: 50µl, 100µl

Swiss-Prot: P01562

Host: Rabbit

Reactivity: Human, Rat, Mouse

Applications: WB IF

Background: Interferons (IFNs) appear both locally and systematically early after viral infection and participate in limiting the spread of infection. They also affect cell differentiation, growth, surface antigen expression and immunoregulation. There are three naturally occurring interferons: α, β and γ. IFN-α is derived from lymphoblastic tissue and has a number of therapeutic applications in the treatment of various human cancers and diseases of viral origin. Recombinant IFN-α from both natural and synthetic genes binds to a common cell surface receptor and induces antiviral activity in a variety of cell lines. When binding to discrete cell surface receptors on target cells, IFN-α induces rapid changes in Jak/Stat phosphorylation, which initiates the Jak/Stat signaling pathway. IFN-α signaling also involves production of DAG without an increased intracellular free calcium concentration and the subsequent activation of calcium-independent isoforms of PKC (β and ε). All IFN-α signaling pathways lead to final alterations of gene expression, which mediate their pleiotropic biologic activities.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: IFN-α polyclonal antibody detects endogenous levels of IFN-α protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of IFN-α polyclonal antibody at 1:500 dilution
Lane 1: The testis tissue lysate of Mouse (20ug)
Lane 2: The spleen tissue lysate of Rat (20ug)
Lane 3: A549 whole cell lysate (40ug)
Lane 4: U-87MG whole cell lysate (40ug) Lane 5: C6 whole cell lysate (40ug)
Lane 6: BV2 whole cell lysate (40ug)
Lane 7: PC3 whole cell lysate (40ug), Immunofluorescence analysis of A549 cells using IFN-α antibody at dilution of 1:50.

Bioworld Molecular Weight: ~ 19 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Aldehyde oxidase; Aldehyde oxidase 1; Azaheterocycle hydroxylase; AOX1

Immunogen: Synthetic peptide, corresponding to Human IFN-α.

Conjugate: Unconjugated

Modification: Unmodified