Ecat1 Polyclonal Antibody

Product: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Sizes: 50µl, 100µl

Catalogue Numbers: BS65765-50, BS65765-100

Swiss-Prot: Q587J8

Host: Rabbit

Ecat1 Polyclonal Antibody-BS65765

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Reactivity: Human,

Applications: IHC-P

All Applications: IHC-P=1:100-500

Background: ECAT1 (ES cell-associated transcript 1 protein) is a 217 amino acid protein that belongs to the KHDC1 family. The ECAT1 protein contains an atypical KH domain with amino acid changes at critical sites, suggesting that it may not bind RNA. Expression of ECAT1 appears to be maximal in germinal vesicle oocytes, it tails off through metaphase II oocytes and is undetectable following the completion of the oocyte to embryo transition. Specifically expressed in the oocytes, recent studies suggest that ECAT1 may function as a regulator of genomic imprinting in the oocyte. Defects in ECAT1 are the cause of hydatidiform mole recurrent type 2 (HYDM2), a disorder characterized by excessive trophoblast development that produces a growing mass of tissue inside the uterus at the beginning of a pregnancy. HYDM2 leads to abnormal pregnancies with no embryo, and cystic degeneration of the chorionic villi.

Purification and Purity: affinity purified by Protein A

Specificity: Ecat1 Polyclonal Antibody detects endogenous levels of Ecat1 protein.

Bioworld Molecular Weight: 24kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Paraformaldehyde-fixed, paraffin embedded (human endometrial carcinoma)Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Ecat1) Polyclonal Antibody, Unconjugated (bs-13596R) at 1:200 overnight at 4°C, Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma)Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Ecat1) Polyclonal Antibody, Unconjugated (bs-13596R) at 1:200 overnight at 4°C

Alternative Name: C6orf221; Chromosome 6 open reading frame 221; ES cell-associated transcript 1 protein; HYDM2; KHD3L_HUMAN; KHDC3-like protein; KHDC3L.聽

Immunogen: KLH conjugated synthetic peptide derived from human Ecat1:1-100/217

Modification: Unmodified

Conjugate: Unconjugated