Cleaved Caspase-9 P37 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS80387-50, BS80387-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P55211

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF

All Applications: WB,1:500 - 1:1000 | IHC,1:100 - 1:200 | IF,1:100 - 1:500

Background: This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein can undergo autoproteolytic processing and activation by the apoptosome, a protein complex of cytochrome c and the apoptotic peptidase activating factor 1; this step is thought to be one of the earliest in the caspase activation cascade. This protein is thought to play a central role in apoptosis and to be a tumor suppressor. Alternative splicing results in multiple transcript variants.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Cleaved Caspase-9 P37 polyclonal antibody detects endogenous levels of Cleaved Caspase-9 P37 protein.

Bioworld Molecular Weight: ~ 37 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Caspase 9 p37 expression in A549 (A), HEK293T (B) whole cell lysates., Immunohistochemical analysis of Caspase 9 p37 staining in human lung cancer formalin fixed paraffin embedded tissue section., Immunofluorescent analysis of Caspase 9 p37 staining in HeLa cells.

Alternative Name: CASP9; APAF-3; APAF3; ICE-LAP6; MCH6; PPP1R56; caspase-9; casp9; Caspase 9

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Caspase 9 p37.

Conjugate: Unconjugated

Modification: Unmodified