Recombinant Human TPBG/5T4 (C-mFc)

Sizes: 10μg, 50μg

Catalogue Numbers: PKSH033920-10, PKSH033920-50

Citations, Manuals and MSDS Available upon request.

Abbreviation: TPBG; 5T4

Target Symptom: 5T4; 5T4AG; M6P1; WAIF1

Research Area: Cancer; Developmental Biology; immunology; Stem cells

Target Species: Human

Expression Host: HEK293 Cells

Fusion Tag: C-mFc

UNIProt ID: Q13641

Accession: Q13641

Background: Trophoblast glycoprotein, also known as TPBG and 5T4, it belongs to the LRR family of proteins. The C-terminus of LRR N-terminal cap (LRRNT) and LRR 1 are essential for the inhibition of the Wnt signaling pathway. TPBG may function as an inhibitor of Wnt/beta-catenin signaling by indirectly interacting with LRP6 and blocking Wnt3a-dependent LRP6 internalization. It expressed by all types of trophoblasts as early as 9 weeks of development. In adult tissues, the expression is limited to a few epithelial cell types but is found on a variety of carcinoma.

Sequence: Ser32-Ser355

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 61.7 kDa

Observed MW: 80-110 kDa

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Research Use Only