NUAK1 Polyclonal Antibody

Sizes: 60μL, 120μL, 200μL

Catalogue Numbers: E-AB-67108-60, E-AB-67108-120, E-AB-67108-200

Citations, Manuals and MSDS Available upon request.

Abbreviation: NUAK1

Target Synonym: NUAK1; ARK5

Research Areas: Cancer, Signal Transduction

Conjugation: Unconjugated

Host: Rabbit

Species Reactivity: Human, Rat

Application: WB

Isotype: IgG

Clonality: Polyclonal

UNIProt ID: O60285

Background: NUAK family SNF1-like kinase 1also known asAMPK-related protein kinase 5(ARK5) is anenzymethat in humans is encoded by theNUAK1gene. Serine/threonine-protein kinase involved in various processes such as cell adhesion, regulation of cell ploidy and senescence, cell proliferation and tumor progression. Phosphorylates ATM, CASP6, LATS1, PPP1R12A and p53/TP53. Acts as a regulator of cellular senescence and cellular ploidy by mediating phosphorylation of 'Ser-464' of LATS1, thereby controlling its stability. Controls cell adhesion by regulating activity of the myosin protein phosphatase 1 (PP1) complex. Acts by mediating phosphorylation of PPP1R12A subunit of myosin PP1: phosphorylated PPP1R12A then interacts with 14-3-3, leading to reduced dephosphorylation of myosin MLC2 by myosin PP1.

Concentration: 1mg/mL

Immunogen: Recombinant fusion protein of human NUAK1 (NP_055655.1).

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification Method: Affinity purification

Dilution: WB 1:200-1:2000

Calculated MW: 23kDa/74kDa

Observed MW: 74kDa

Storage: -20°C/One year. Avoid freeze / thaw cycles.

Shipping: Ice bag

Research Use Only