BLNK Monoclonal Antibody

Catalogue Numbers: MB67162-50, MB67162-100

Sizes: 50µl, 100µl

Product: Mouse IgM. Supplied in crude ascites with 0.01% sodium azide.

Swiss-Prot: Q8WV28

Host: Mouse

Reactivity: Human

Applications: WB

All Applications: WB (1/500 - 1/4000)

Background: B cell linker protein (BLNK), also known as SLP-65 or BASH, is an adaptor molecule that plays key roles in B cell activation and B cell antigen receptor (BCR) engagement. BLNK acts at the interface between BCR-associated Syk and downstream signaling cascades. BLNK has multiple SH2 binding motifs (YXXP) at its amino terminus and an SH2 domain at its carboxy terminus. After BCR ligation, BLNK is phosphorylated by Syk at multiple YXXP motifs including Tyr72, Tyr84, Tyr96, and Tyr178 . These phosphorylated motifs provide docking sites for signaling molecules, such as BTK, PLCγ, and Vav. These signaling molecules bind to BLNK through their SH2 domains and together activate downstream signaling pathways. Through its SH2 domain, BLNK can also interact with tyrosine-phosphorylated targets, such as HPK1, thereby recruiting them to the BCR complex for signaling.

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Recognizes endogenous levels of BLNK protein.

Extra Notes: Western blot analysis of BLNK expression in CEM (A) whole cell lysates.

Bioworld Molecular Weight: ~ 68 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: BASH; SLP65; B-cell linker protein; B-cell adapter containing a SH2 domain protein; B-cell adapter containing a Src homology 2 domain protein; Cytoplasmic adapter protein; Src homology 2 domain-containing leukocyte protein of 65 kDa; SLP-65

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human BLNK. The exact sequence is proprietary.

Conjugate: Unconjugated

Modification: Unmodified