XBP1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS91458-50, BS91458-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P17861(Human) O35426(Mouse) Q9R1S4(Rat)

Host: Rabbit

Reactivity: Human

Applications: WB, IHC ICC/IF FC

All Applications: WB:1:500-1:1,000
ICC:1:100-1:500
IHC:1:50-1:200
FC:1:50-1:100

Background: The X-box binding protein-1 (XBP-1 or hXBP-1), also designated tax-responsive element-binding protein 5 (TREB5) in mouse and human, or hepatocarcinogenesis-related transcription factor (HTF) in rat, belongs to the basic region/leucine zipper (bZIP) family of transcription factors. XBP-1 was first characterized as a protein that binds to the HLA-DRα promoter in B cells. XBP-1 recognizes the cAMP responsive element (CRE) in enhancers of human T cell leukemia virus and major histocompatibility complex class II genes and activates transcription of these genes. It is expressed at high levels in developing bone and its levels are modulated during osteoblast development, suggesting a role in regulation of expression of osteoblast-specific genes. In addition to binding to CRE sequences, XBP-1 has been shown to bind to TPA response elements (TREs).

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: XBP1 polyclonal antibody detects endogenous levels of XBP1 protein.

Bioworld Molecular Weight: 40 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of XBP1 on different cell lysates using anti-XBP1 antibody at 1/1,000 dilution.
Positive control:
Lane 1: HepG2
Lane 2: 293T, ICC staining XBP1 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: XBP-1, TREB-5, Tax responsive element binding protein 5, Tax-responsive element-binding protein 5, TREB5, X box binding protein 1, X box binding protein 2, X-box-binding protein 1, XBP 1, XBP-1, XBP1, XBP1, XBP2,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified