AQP2 (R252) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS1718-50, BS1718-100

Sizes: 50µl, 100µl

Swiss-Prot: P41181

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

Background: Many isoforms of aquaporin have been identified in mammals, designated AQP0 through AQP10. Aquaporins are widely distributed and it is not uncommon for more than one type of AQP to be present in the same cell. Although most aquaporins are only permeable to water, AQP3, AQP7, AQP9 and one of the two AQP10 transcripts are also permeable to urea and glycerol. AQP2 is the only water channel that is activated by vasopressin to enhance water reabsorption in the kidney collecting duct. Aquaporins are involved in renal water absorption, generation of pulmonary secretions, lacrimation, and the secretion and reabsorption of cerebrospinal fluid and aqueous humor.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: AQP2 (R252) polyclonal antibody detects endogenous levels of AQP2 protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of AQP2 (R252) pAb at 1:500 dilution
Lane 1: HEK293T whole cell lysate (40µg)
Lane 2: Hela whole cell lysate (40µg)
Lane 3: The Kidney tissue lysate of Rat (40µg)
Lane 4: CT-26 whole cell lysate (40µg), Immunohistochemistry (IHC) analyzes of AQP2 (R252) pAb in paraffin-embedded human colorectal carcinoma tissue at 1:50.

Bioworld Molecular Weight: ~ 26 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Aquaporin-2; AQP-2; ADH water channel; Aquaporin-CD; AQP-CD; Collecting duct water channel protein; WCH-CD; Water channel protein for renal collecting duct; AQP2

Immunogen: Synthetic peptide, corresponding to amino acids 230-280 of Human AQP2.

Conjugate: Unconjugated

Modification: Unmodified