GABRA5 Polyclonal Antibody

Sizes: 50 µl, 100 µl

Catalogue Numbers: BS90550-50, BS90550-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P31644

Host: Rabbit

Reactivity: Mouse, Rat

Applications: WB

All Applications: WB,1:1000 - 1:4000

Background: GABA is the major inhibitory neurotransmitter in the mammalian brain where it acts at GABA-A receptors, which are ligand-gated chloride channels. Chloride conductance of these channels can be modulated by agents such as benzodiazepines that bind to the GABA-A receptor. At least 16 distinct subunits of GABA-A receptors have been identified. Transcript variants utilizing three different alternative non-coding first exons have been described.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using GABRA5 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 3s.

Bioworld Molecular Weight: 52kDa

Specificity: Polyclonal Antibodies

Alternative Name: GABRA5

Immunogen: Recombinant fusion protein of human GABRA5(NP_000801.1).

Conjugate: Unconjugated

Modification: Unmodified