Desmoplakin Polyclonal Antibody

Product: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Catalogue Numbers: BS67329-50, BS67329-100

Sizes: 50µl, 100µl

Swiss-Prot: P15924

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

Background: Desmosomes are a class of intracellular junction that tightly link adjacent cells in mechanically stressed tissues such as the epithelium and myocardium . They derive their characteristic strength from the protein Desmoplakin, which acts as a tether by binding the cytoplasmic component of the Desmosome at it’s N-terminus while its C-terminus is anchored to the intermediate-filament cytoskeleton . This association mitigates the impact of mechanical forces on the desmosome by distributing them throughout the cytoskeleton and tissue . Desmoplakin is essential for normal desmosomal adhesion ; defects can result in pathologies that include cardiomyopathy , keratoderma , or the skin blistering disease Epidermolysis bullosa .
Phospho-Desmoplakin (Ser165/166) Antibody is directed against a site that was identified at Cell Signaling Technology (CST) using PTMScan®, our LC-MS/MS platform for modification site discovery. Please visit PhosphoSitePlus®, a modification site knowledgebase developed and maintained by CST, at www.phosphosite.org for more information.

Product: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Recognizes endogenous levels of Desmoplakin protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Desmoplakin expression in MCF7 (A), Hela (B), mouse heart (C) whole cell lysates., Immunohistochemical analysis of Desmoplakin staining in human stomach cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Bioworld Molecular Weight: ~ 331 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Desmoplakin; DP; 250/210 kDa paraneoplastic pemphigus antigen

Immunogen: KLH-conjugated synthetic peptide of human Desmoplakin

Conjugate: Unconjugated

Modification: Unmodified