SPTBN5 Polyclonal Antibody

Catalogue Numbers: BS61279-50, BS61279-100

Sizes: 50µl, 100µl

Alternative Name: Spectrin beta chain, non-erythrocytic 5; Beta-V spectrin; SPTBN5; BSPECV; HUBSPECV; HUSPECV

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9NRC6

Host: Rabbit

Reactivity: Human

Applications: WB

All Applications: WB: 1:500~1:1000

Background: Whole-exome sequencing (WES) identified 46 non-synonymous and 13 synonymous somatic single-nucleotide polymorphisms (SNPs) and one frameshift deletion in the coding regions. Specifically, mutations of CDKN2A, TP53, SPTBN5, NOTCH2, and FAM136A were found in the databases. Copy number aberration (CNA) analysis revealed that the cell line loses chromosome 3p and 9p, but lacks amplification of 3q and 11q (as does HPV-negative, smoking-unrelated OTSCC). It also exhibits four distinctive focal amplifications in chromosome 19p, containing 131 genes without SNPs. Particularly, 52 genes showed >3- to 4-fold amplification and could be potential oncogenic drivers.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: SPTBN5 pAb detects endogenous levels of SPTBN5 protein.

Bioworld Molecular Weight: ~ 416 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of SPTBN5 pAb at 1:500 dilution
Lane 1: HEK293T whole cell lysate(40ug)
Lane 2: SGC7901 whole cell lysate(40ug)
Lane 3: Panc1 whole cell lysate(40ug)

Immunogen: Synthetic peptide, corresponding Human SPTBN5.

Conjugate: Unconjugated

Modification: Unmodified