CD71 Monoclonal Antibody

Catalogue Numbers: MB66783-50, MB66783-100

Sizes: 50µl, 100µl

Product: Mouse IgM. Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Swiss-Prot: P02786

Host: Mouse

Reactivity: Human

Applications: WB

All Applications: WB (1/500 - 1/1000)

Background: CD71, also known as the transferrin receptor (TFR), is a type II membrane glycoprotein that exists as a disulfide-linked homodimer of two identical subunits. CD71 binds to two molecules of transferrin and a serum iron-transport protein, and directs the cellular uptake of iron via receptor-mediated endocytosis. CD71 is expressed, typically at high levels, on all proliferating cells, reticulocytes and erythroid precursors. It is not expressed on resting leukocytes, but is upregulated upon activation of lymphocytes, monocytes and macro-phages. CD71 is also found on most dividing cells and on brain endothelium. A second transferrin receptor, TFR2, also mediates the uptake of transferrin-bound iron. TFR2 is a two-subunit homodimer and is highly expressed in liver as well as in hepatocytes and erythroid precursors. Mutations in the TFR2 gene result in hereditary hemochromatosis type III (HFE3), an iron overloading disorder predominant in Caucasians.

Purification and Purity: This antibody is purified through a protein G column.

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Recognizes endogenous levels of CD71 protein.

Extra Notes: Western blot analysis of CD71 expression in HT1080 (A), Hela (B) whole cell lysates.

Bioworld Molecular Weight: ~ 101 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: Transferrin receptor protein 1; TR; TfR; TfR1; Trfr; T9; p90; CD71

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CD71. The exact sequence is proprietary.

Conjugate: Unconjugated

Modification: Unmodified