Recombinant 2019-nCoV S-trimer Protein (C-6His)

Size: 50ug

Catalogue Number: EPT040-50

Citations, Manuals and MSDS Available upon request.

Background: The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. Most notable is severe acute respiratory syndrome (SARS). The severe acute respiratory syndrome-coronavirus (SARS-CoV) spike (S) glycoprotein alone can mediate the membrane fusion required for virus entry and cell fusion. It is also a major immunogen and a target for entry inhibitors. It's been reported that 2019-nCoV can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Alternate Names: 2019-nCov S protein; 2019-nCoV Spike glycoprotein; 2019-nCoV S glycoprotein

Description: Recombinant SARS-CoV-2 S-trimer Protein is produced by our Mammalian expression system and the target gene encoding Cys15-Gln1208 is expressed with a 6His tag at the C-terminus.

Accession: QHD43416.1

Molecular Weight: 136.6kDa

Apparent Molecular Weight: 170-220kDa, reducing conditions

Storage Condition: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Purity: Greater than 95% as determined by reducing SDS-PAGE.

Biological Activity: Anti-2019-nCoV S-hIgG1 Antibody (8A5) (Cat#DA034) can block Human ACE-2-Avi-His (Cat#CY51) and 2019-nCoV S-trimer Protein-His (Cat#DRA49) interaction, the IC50 for this effect is 25 ng/ml.

Delivery Condition: The product is shipped on dry ice pack. Upon receipt, store it immediately at the temperature listed below.

Storage: Reconstituted protein solution should be stored at ≤ -20°C.

Research Use Only