DTSF/HB-EGF Polyclonal Antibody Sizes: 50µl, 100µl Catalogue Numbers: BS65575-50, BS65575-100 Product: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Swiss-Prot: Q99075 Host: Rabbit Reactivity: Human, Mouse, Rat, Pig, Cow,Sheep, Applications: IHC-P All Applications: IHC-P=1:100-500（石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. Background: Heparin-binding epidermal growth factor-like growth factor (HB-EGF) is a 22kDa O-glycosylated protein that is a potent mitogen and chemoattractant for vascular smooth muscle cells, fibroblasts and epithelial cells but not endothelial cells. The natural protein has an apparent molecular mass of 19-23 kDa and exists in multiple forms as a result of heterogeneous O-glycosylation and/or Nterminal truncation. HB-EGF is synthesized as a membrane-anchored precursor(proHB-EGF) that is proteolytically cleaved to release the soluble mature growth factor. The two forms are active as juxtacrine and paracrine/autocrine growth factors respectively.HB-EGF activates two EGF receptor subtypes, HER1/ErbB1 and HER4 and binds to heparan sulfate proteoglycan. Purification and Purity: affinity purified by Protein A Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity: DTSF/HB-EGF polyclonal Antibody detects endogenous levels of DTSF/HB-EGF protein. Bioworld Molecular Weight: ~21kDa Note: For research use only, not for use in diagnostic procedure. Extra Notes: Paraformaldehyde-fixed, paraffin embedded (Human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes;Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DTSF) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C. Alternative Name: HB EGF; Diphtheria toxin receptor; DT R; DT-R; DTR; DTS; DTSF; HB-EGF; HBEGF; HBEGF_HUMAN; HEGFL; heparin binding EGF like growth factor; Heparin binding epidermal growth factor; Heparin binding epidermal growth factor like growth factor; Heparin-binding EGF-like growth factor; Proheparin binding EGF like growth factor. Immunogen: KLH conjugated synthetic peptide derived from human DTSF/HB-EGF:20-62/208 Conjugate: Unconjugated Modification: Unmodified