Estrogen Receptor beta Monoclonal Antibody Catalogue Numbers: MB67166-50, MB67166-100 Sizes: 50µl, 100µl Product: Mouse IgG1. Supplied in crude ascites with 0.01% sodium azide. Swiss-Prot: Q92731 Host: Mouse Reactivity: Human Applications: WB All Applications: WB (1/500 - 1/2000) Background: Estrogen receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of ligand-activated transcription factors. Estrogen receptors,including ERα and ERβ,contain DNA binding and ligand binding domains and are critically involved in regulating the normal function of reproductive tissues. They are located in the nucleus, though some estrogen receptors associate with the cell surface membrane and can be rapidly activated by exposure of cells to estrogen. ERα and ERβ have been shown to be differentially activated by various ligands. Receptor-ligand interactions trigger a cascade of events, including dissociation from heat shock proteins, receptor dimerization, phosphorylation and the association of the hormone activated receptor with specific regulatory elements in target genes. Evidence suggests that ERα and ERβ may be regulated by distinct mechanisms even though they share many functional characteristics. Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity: Recognizes endogenous levels of Estrogen Receptor beta protein. Extra Notes: Western blot analysis of Estrogen Receptor beta expression in K562 (A) whole cell lysates. Bioworld Molecular Weight: ~ 55 kDa Note: For research use only, not for use in diagnostic procedure. Alternative Name: ESTRB; NR3A2; Estrogen receptor beta; ER-beta; Nuclear receptor subfamily 3 group A member 2 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Estrogen Receptor beta. The exact sequence is proprietary. Conjugate: Unconjugated Modification: Unmodified