MDC1 (phospho Ser513) rabbit pAb

Sizes: 50μL, 100μL

Catalogue Numbers: ES8081-50, ES8081-100

Citations, Manuals and MSDS Available upon request.

Background: The protein encoded by this gene contains an N-terminal forkhead domain, two BRCA1 C-terminal (BRCT) motifs and a central domain with 13 repetitions of an approximately 41-amino acid sequence. The encoded protein is required to activate the intra-S phase and G2/M phase cell cycle checkpoints in response to DNA damage. This nuclear protein interacts with phosphorylated histone H2AX near sites of DNA double-strand breaks through its BRCT motifs, and facilitates recruitment of the ATM kinase and meiotic recombination 11 protein complex to DNA damage foci. [provided by RefSeq, Jul 2008],

Alternate Name: MDC1; KIAA0170; NFBD1; Mediator of DNA damage checkpoint protein 1; Nuclear factor with BRCT domains 1

Source: Rabbit

Applications: IHC; IF; ELISA

Dilution: Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.

Reactivity: Human; Rat; Mouse;

Immunogen: The antiserum was produced against synthesized peptide derived from human MDC1 around the phosphorylation site of Ser513. AA range:479-528

Storage and Stability: -20°C/1 year

Clonality: Polyclonal

Isotype: IgG

Concentration: 1 mg/ml

Human Gene ID: 9656

Human SWISS Prot NO: Q14676

Subcellular Location: Nucleus. Chromosome. Associated with chromatin. Relocalizes to discrete nuclear foci following DNA damage, this requires 'Ser-139' phosphorylation of H2AX. Colocalizes with APTX at sites of DNA double-strand breaks.

Research Use Only