Recombinant Human EphA7/EHK3 Protein (His Tag)(Active)

Sizes: 10μg, 50μg

Catalogue Numbers: PKSH033689-10, PKSH033689-50

Citations, Manuals and MSDS Available upon request.

Abbreviation: EphA7; EHK3

Target Symptom: Ephrin Type-A Receptor 7; EPH Homology Kinase 3; EHK-3; EPH-Like Kinase 11; EK11; hEK11; EPHA7; EHK3; HEK11

Research Area: Cardiovascular; Neuroscience

Target Species: Human

Expression Host: HEK293 Cells

Application: ELISA

Fusion Tag: C-His

UNIProt ID: Q15375

Accession: Q15375

Background: Ephrin Type-A Receptor 7 (EPHA7) is a single-pass type I membrane protein which belongs to the Eph family of transmembrane receptor tyrosine kinases. It contains two fibronectin type-III domains, one protein kinase domain and one SAM (sterile alpha motif) domain. EPHA7 is a receptor for members of the ephrin-A family. Eph receptors are largely expressed throughout the ectoderm, mesoderm, and endoderm of vertebrate embryos. EPHA7 functions as a repulsive guidance molecule during the targeting of retinal axons to the superior colliculus and of neocortical axons to the thalamus. EPHA7 is expressed at a substantial level in most human lung cancers. The high expression of EPHA7 protein may participate in the malignancy transformation, invasion progression and metastasis of primary hepatocellular carcinoma. EPHA7 may involve in smoking related lung carcinogenesis.

Activity: Immobilized Human EphA7-His at 2μg/ml(100 μl/well) can bind Human EFNA4-Fc-6His(Cat: PKSH032392). The ED50 of Human EphA7-His is 1. 5190 ug/ml.

Sequence: Gln28-Ile556

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.2.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 60.19 kDa

Observed MW: 72 kDa

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Research Use Only