PI4KB Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS80020-50, BS80020-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9UBF8

Host: Rabbit

Reactivity: Mouse, Rat

Applications: WB, IF/ICC

All Applications: WB,1:500 - 1:2000 | IF/ICC,1:50 - 1:200

Background: Phosphorylates phosphatidylinositol (PI in the first committed step in the production of the second messenger inositol-1,4,5,-trisphosphate (PIP. May regulate Golgi disintegration/reorganization during mitosis, possibly via its phosphorylation. Involved in Golgi-to-plasma membrane trafficking (By similarity.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Unmodification

Bioworld Molecular Weight: 91KDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Mouse thymus, using PI4KB antibody at 1:2590 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 0.8s., Immunofluorescence analysis of C6 cells using PI4KB antibody at dilution of 1:100. Blue: DAPI for nuclear staining., Immunofluorescence analysis of L929 cells using PI4KB antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

Alternative Name: PI4KB; NPIK; PI4K-BETA; PI4K92; PI4KBETA; PI4KIIIBETA; PIK4CB

Immunogen: Recombinant fusion protein of human PI4KB(NP_001185702).

Conjugate: Unconjugated

Modification: Unmodified