Porcine bFGF/FGF2 (Basic Fibroblast Growth Factor) ELISA Kit

Sizes: 24T, 48T, 96T

Catalogue Numbers: E-EL-P0744-24, E-EL-P0744-48, E-EL-P0744-96

Citations, Manuals and MSDS Available upon request.

Abbreviation: bFGF; FGF2

UNIProt ID: Q7YRN5

Target Synonyms: FGF-2; B-FGF; BFGF; FGFB; HBGF-2; prostatropin; heparin-binding growth factor 2; FGF-β;

Target Species: Porcine

Application: ELISA

Usage: This ELISA kit applies to the in vitro quantitative determination of Porcine bFGF/FGF2 concentrations in Serum, plasma and other biological fluids.

Tested Sample Types: Serum, Plasma and other biological fluids

Type: Sandwich

Assay Time: 4.5h

Detection Type: Colormetric

Sensitivity: 9.38 pg/mL

Detection Range: 15.63-1000 pg/mL

Test Principle: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Porcine bFGF/FGF2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Porcine bFGF/FGF2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Porcine bFGF/FGF2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Porcine bFGF/FGF2. You can calculate the concentration of Porcine bFGF/FGF2 in the samples by comparing the OD of the samples to the standard curve.

Cross Reactivity: This kit recognizes Porcine bFGF/FGF2 in samples. No significant cross-reactivity or interference between Porcine bFGF/FGF2 and analogues was observed

Recovery Rate: 80%-120%

Precision: Both intra-CV and inter-CV are < 10%.

Storage: 2-8℃/-20℃, 12 months

Shipping: ice bag