Wilms Tumor Protein Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS65971-50, BS65971-100

Product: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Swiss-Prot: P19544

Host: Rabbit

Reactivity: Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Sheep,

Applications: IHC-P, FCM，

All Applications: IHC-P=1:100-500 Flow-Cyt=1ug/test

Background: Transcription factor that plays an important role in cellular development and cell survival. Regulates the expression of numerous target genes, including EPO. Plays an essential role for development of the urogenital system. Recognizes and binds to the DNA sequence 5'-CGCCCCCGC-3'. It has a tumor suppressor as well as an oncogenic role in tumor formation. Function may be isoform-specific: isoforms lacking the KTS motif may act as transcription factors. Isoforms containing the KTS motif may bind mRNA and play a role in mRNA metabolism or splicing. Isoform 1 has lower affinity for DNA, and can bind RNA.

Purification and Purity: affinity purified by Protein A

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Wilms Tumor Protein Polyclonal Antibody detects endogenous levels of Wilms Tumor Protein protein.

Bioworld Molecular Weight: 55kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-WT-1/Wilms Tumor Protein Polyclonal Antibody, Unconjugated 1:200, overnight at 4°C, Paraformaldehyde-fixed, paraffin embedded (Mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Wilms Tumor Protein) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, Paraformaldehyde-fixed, paraffin embedded (Rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Wilms Tumor Protein) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C

Alternative Name: WIT 2; WT 1; AWT1; FWT1; GUD; NPHS4; WAGR; Wilms tumor 1; Wilms Tumor; Wilms tumor protein; Wilms' tumor gene; Wilms' tumor protein; WIT2; WT; WT1; WT-1; WT1_HUMAN; WT33.

Immunogen: KLH conjugated synthetic peptide derived from human Wilms Tumor Protein:301-400/449

Conjugate: Unconjugated

Modification: Unmodified