Recombinant Human GAPDH Protein (His Tag)

Size: 100μg

Catalogue Numbers: PKSH031882-100

Citations, Manuals and MSDS Available upon request.

Abbreviation: GAPDH

Target Synonym: G3PD; GAPD; HEL-S-162eP

UNIProt ID: P04406

Research Areas: Signal Transduction; Cancer; Isotypeloading control; metabolism;

Target Species: Human

Expression Host: E.coli

Fusion Tag: N-His

Accession: P04406

Background: Glyceraldehyde 3-phosphate dehydrogenase (GAPDH or G3PDH) is an enzyme of about 37kDa that is consisdered as a cellular enzyme involved in glycolysis. It catelyzes the sixth step of glycolysis. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a pleiotropic enzyme that is overexpressed in apoptosis and in several human chronic pathologies. Its role as a mediator for cell death has also been highlighted. A recent report suggests that GAPDH may be genetically associated with late-onset of Alzheimer's disease. Besides, deprenyl, which has originally been used as a monoamine oxidase inhibitor for Parkinson's disease, binds to GAPDH and displays neuroprotective actions.

Sequence: Met 1-Glu 335

Purity: > 93 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from sterile 50mM Tris, 30% glycerol, pH 7.5
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: Please contact us for more information.

Calculated MW: 38 kDa

Observed MW: 38 kDa

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Research Use Only